Direct detection of palmitoyl protein/peptide by mass spectrometry
نویسندگان
چکیده
Palmitoylation is a lipid modification of proteins with the covalent attachment of a palmitoyl group to a cysteine residue through a thioester linkage. Direction detection of palmitoylation is challenging because the thioester linkage is labile and palmitoyl group losses could occur during sample preparation if care is not taken. In addition, palmitoyl group dramatically increases the hydrophobicity of peptides, which makes them hard to elute using regular LC method for peptide. Here we present a sample preparation method and optimized LC-MS condition for direct identification of palmitoyl protein/peptide.
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